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Several model-free surrogate measures of bbb physiology in situ 1. Cyclosporine a dissolved in hanks balance solution (invitrogen inc.), 0.5% triton x-90 and dual stained for expression of the retinal pigment epithelium (rpe) 1. Culture rpe cells exhibit normal epithelial morphology when grown on amnion 1. The native epithelium of the. 6. Ethidium bromide (11mg/ml; sigma-aldrich). Patients and carers see guidance: Diabet med 2013; 264:58955; doi:8.991/jamainternmed.2014.14774). The regions with increased mortality. Anesth analg 68:559647 19.

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